The Michaelis– Menten parameters are affected by systematic errors in initial substrate concentration, product concentration, and reaction time. The fitted curve deviates little from the data, making it difficult to detect these errors.
Is a Michaelis-Menten plot accurate?
For small system volumes, the accuracy of the approximation decreases. The steady-state concentration of S is the same for both the MM and MA systems in the continuum ODE model.
Why is it difficult to accurately estimate Km and Vmax values from a Michaelis-Menten plot?
It is difficult to fit the best hyperbola through the experimental points.
Why is Lineweaver Burk more accurate than Michaelis?
Lineweaver-Burke plot has two advantages over the Michaelis- Menten plot, one of which is that it gives a more accurate estimate of V max. inhibition
Which experimental condition is not necessary to achieve reliable data for Michaelis-Menten enzyme kinetics?
The answer is D because once the reaction reaches equilibrium, measurement of Vo will be impossible and the data will look the same regardless of the concentration.